|
KMID : 0869020060090010040
|
|
Journal of Korean Orthopaedic Research Society
2006 Volume.9 No. 1 p.40 ~ p.47
|
|
|
Role of Tumor Necrosis Factor Alpha, Interleukin 8 and Dexamethasone in the FAK Expression by Human Nucleus Pulposus Cells
|
|
Yoon Jin-Ho
Lee Ju-Hong
|
|
|
Abstract
|
|
|
Purpose: Human nucleus pulposus cells from intervertebral disc specimens were cultured to study the effects of TNF-alphaand IL-8 on the FAK expression by these cells. The effect of co-stimulation with dexamethasone on the FAK expression by nucleus pulposus cells was also studied.
Materials and Methods:The FAK expression in cultured human nucleus pulposus cells relation to TNF-alpha, IL-8 and dexamethasone was assessed by performing western blot.
Results:The activated FAK expression was up-regulated by treatments of TNF-alpha and IL-8. Dexamethasone attenuated the cytokine-induced FAK expression. The effects of inflammatory cytokines on the FAK expression were found to be concentration dependent with greater correlation shown by IL-8 than by TNF-alpha.
Conclusion:The FAK expression of human nucleus pulposus cells was up-regulated by TNF-alpha and IL-8 stimulation and it was attenuated by the co-administration of dexamethasone.
|
|
|
KEYWORD
|
|
|
Human uncleus pulposus, Focal adhesion kinase, Tumor necrosis factor alpha, Interleukin-8
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|